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Ancient greeks were the first to use animals to practice procedures before using them on humans
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Nafis gave an accurate description of how blood circulates through animals.
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Robert Boyle tested animals reliance to air by cutting off their air supply and seeing how long it would take for the animal to take their last breath.
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A shock was used to on frog which produced a twitch. The Start of using electricity on human organs.
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Ivan Pavlov used classical conditioning on dogs.
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Edgar Adrain developed a theory of neural communication for action potential. Which is all about the nerves and how they work.
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The first cloned animal was dolly the Sheep.
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Using animals as an experiment continues today. It is used as research that is used to solve medical problems in where there is no useful medical alternatives available.