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Haberlandt proposed concept of in vitro cell culture
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Hannig cultured embryos from several cruciferous species
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Kolte and Robbins successfully cultured root and stem tips respectively
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Went discovered first plant growth hormone –Indole acetic acid
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White introduced vitamin B as growth supplement in tissue culture media for tomato root tip
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Gautheret, White and Nobecourt established endless proliferation of callus cultures.
Successful continuously growing cambial cultures of carrot and tobacco. -
Overbeek was first to add coconut milk for cell division in Datura
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Ball raised whole plants of Lupinus by shoot tip culture
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Muir was first to break callus tissues into single cells
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Skoog and Miller discovered kinetin as cell division hormone
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Skoog and Miller gave concept of hormonal control (auxin: cytokinin) of organ formation.
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Cocking was first to isolate protoplast by enzymatic degradation of cell wall
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Bergmann filtered cell suspension and isolated single cells by plating
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Murashige and Skoog developed MS medium with higher salt concentration
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Steward demonstrated totipotency by regenerating carrot plants from single cells of tomato
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Guha and Maheshwari produced first haploid plants from pollen grains of Datura
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Carlson produced first interspecific hybrid of Nicotiana by protoplast fusion
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Peter Farnum et al.
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Horsh et al developed transgenic tobacco by transformation with Agrobacterium
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Automated mass propagation with organogenesis and embryogenesis. Levi R. et al., Biotechnol., 6: 1035
