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Concept
Haberlandt proposed concept of in vitro cell culture -
Cultured embryos
Hannig cultured embryos from several cruciferous species -
Culture of root and stem tips
Kolte and Robbins successfully cultured root and stem tips respectively -
Discovered the first PGR
Went discovered first plant growth hormone –Indole acetic acid -
Vitamin B as growth supplement
White introduced vitamin B as growth supplement in tissue culture media for tomato root tip -
Callus cultures
Gautheret, White and Nobecourt established endless proliferation of callus cultures.
Successful continuously growing cambial cultures of carrot and tobacco. -
Coconut milk for cell division
Overbeek was first to add coconut milk for cell division in Datura -
Raised whole plants
Ball raised whole plants of Lupinus by shoot tip culture -
Callus into single cells
Muir was first to break callus tissues into single cells -
Kinetin
Skoog and Miller discovered kinetin as cell division hormone -
Concept of hormonal control
Skoog and Miller gave concept of hormonal control (auxin: cytokinin) of organ formation. -
Embryos regeneration
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Protoplast
Cocking was first to isolate protoplast by enzymatic degradation of cell wall -
Single cells Isolation
Bergmann filtered cell suspension and isolated single cells by plating -
MS medium
Murashige and Skoog developed MS medium with higher salt concentration -
Totipotency
Steward demonstrated totipotency by regenerating carrot plants from single cells of tomato -
Haploid plants
Guha and Maheshwari produced first haploid plants from pollen grains of Datura -
Interspecific hybrid by protoplast
Carlson produced first interspecific hybrid of Nicotiana by protoplast fusion -
Biotechnology of Forest Yield
Peter Farnum et al. -
Transgenic plant
Horsh et al developed transgenic tobacco by transformation with Agrobacterium -
Automated mass propagation
Automated mass propagation with organogenesis and embryogenesis. Levi R. et al., Biotechnol., 6: 1035
