Innovations in the History of Biotechnology

  • Microorganisms and microscopes

    Microorganisms and microscopes

    Robert Hooke coined the term "cells" and Anton Van Leewenhoek observed the first microorganisms because of his invention of the microscope.
  • Inheritance of traits

    Inheritance of traits

    Gregor Mendel discovered the simple laws of inheritance of traits that allowed one to predict the outcome of crosses with certain traits.
  • Microbiology

    Microbiology

    Louis Pasteur described the scientific basis for fermentation, established science of microbiology, and proposed the Germ Theory.
  • Nucleic Acid

    Nucleic Acid

    Johann Mischer found nucleic acid in white blood cells from pus in bandages. Later led scientists to believe DNA was inheritable.
  • Chromosomes

    Chromosomes

    Thomas Morgan discovered that genes were chromosomes.
  • Genetic Material

    Genetic Material

    Fred Griffith using mice, proved that genetic material could be moved from one strain of bacteria to another.
  • Penicilin

    Fleming isolated penicilin from a fungus.
  • Adenine equals thymine and cytosine equals guanine

    Adenine equals thymine and cytosine equals guanine

    Chargaff showed that in DNA the number of units of adenine equals those of thymine and cytosine equaled guanine
  • One gene produces one enzyme

    One gene produces one enzyme

    Beadle and Tatum propesed that "One gene produces one enzyme"
  • Viruses replicate DNA

    Viruses replicate DNA

    Hershey and Chase proved viruses replicated using DNA through their "blender experiment"
  • Double Helix

    Double Helix

    Rosalind Franklin and Maurice Wilkins established through X-Ray crystallography that DNA was indeed a double helix.
  • Structure of DNA

    Structure of DNA

    Watson and Crick discovered the structure of DNA
  • Plasmid DNA

    Plasmid DNA

    Hayes disvovered plasmid DNA, circular pieces of DNA found in bacteria
  • Gene Expression

    Gene Expression

    Walter Gerbert discovered the mechanism of gene expression through his study of messenger RNA.
  • Bacterial restriction enzymes

    Bacterial restriction enzymes

    Arber, Nathans, and Smith discovered bacterial restriction enzymes that cut DNA.
  • 64 Codons

    64 Codons

    Khorana and Nirenburg disvocered the 64 codons that code for the 20 amino acids making up proteins.
  • Vinograd

    Vinograd

    DNA plasmids were isolated and purified by Vingrad
  • Escherichia coli

    Escherichia coli

    Madal and Higa were responsible for the first transformation of the bacterium Escherichia coli.
  • Cloning Experiements

    Cloning Experiements

    Cloning experimetents were conducted by Boyer and Cohen.
  • Glenentech is founded

    Glenentech is founded

    The world's first genetic engineering company.
  • recombinant DNA

    recombinant DNA

    140 scientists met to draw up guidlines for work with recombinant DNA.
  • Sequence DNA

    Sequence DNA

    Sanger and Gilbert found a way to sequence DNA.
  • Botstein idnetified patteren in DNA

    Botstein idnetified patteren in DNA

    Botstein found that one could be identified by the pattern made of one's DNA through a digest by different enzymes.
  • Vitro Fertilization

    Vitro Fertilization

    Loise Joy Brown was born, the first human baby born from vitro fertilization.
  • Synthetic Insulin gene into E. Coli

    Synthetic Insulin gene into E. Coli

    Boyer inserted a synthetic insulin into E. coli
  • Genetically altered life is patented

    Genetically altered life is patented

    The U.S. Supreme Court ruled that genetically altered life forms can be patented. Huge start-up for biotech companies
  • First Transgenic animal

    First Transgenic animal

    Ohio University scientists created the first transgenic animals.
  • Human insulin in bacteria

    Human insulin in bacteria

    Eli Lilly Company placed a human insulin gene inside of bacteria.
  • Agrobacterium-mediated gene transfer

    Agrobacterium-mediated gene transfer

    Schell, Chilton, Van Motagu, Fraley, and Horsch transformed plants with Agrobacterium-mediated gene transfer
  • PCR

    PCR

    Kary Mullins invented polymerase chain reaction to amplify DNA
  • Jefferis applied RFLP in DNA

    Jefferis applied RFLP in DNA

    Profiling of the study if criminal cases
  • Florescence sequencer

    Florescence sequencer

    Sped up labor-intense process
  • Gene Gun

    Genes were moved into organism.
  • NIH recised safety guideines

    NIH recised safety guideines

    NIH revised safety guidelines for recombinant DNA to include plants grown in green houses and animals raised in barns
  • RNA interference

    RNA interference

    Andrew Fire and Craig Mello discovered RNA interference, silencing genes, in the worm C. elegans
  • Monoclonal  Antibody

    Monoclonal Antibody

    Kohler, Milstein, and Jerne used monoclonal antibody technology
  • YACs

    YACs

    Maynard Olson invented "yeast artificial chromosomes" (YACs) which are expression vectors for large proteins.
  • Dolly the Sheep

    Dolly the Sheep

    Ian Wilmut cloned Dolly the sheep from an adult cell of a ewe at the Roslin Institute in Scotland
  • DNA Chip

    DNA Chip

    DNA microarray was developed
  • DNA Sequener

    DNA Sequener

    Dalbiri and Garner invented automated DNA sequencer.
  • Riboswitch

    Riboswitch

    Ron Breaker cointed the term riboswitch for part of an mRNA molecule that can regulate its own activity.
  • Human Genome Project

    Human Genome Project

    Francis Collins and Craig Venter announced the Human Genome Project which provided ability to find genes and gave rise to the sequencing of other genomes.