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Francesco Redi
This well known scientist did an experiment where three glass jars filled with meat were left outside. One of them was in the open air, another with a metal lid and the last one with a breathable layer. With this experiment he demonstrated that only when the meat was in the open air the flies multiplied, since they laid eggs, but that the spontaneous generation that they called was not possible because in the other two jars there was no generation at all. -
Antonie van Leeuwenhoek
This Dutch microbiologist observed microorganisms on various materials through his single-lens microscope in the 1670s. Although he did not directly disprove spontaneous generation, his observations would be used in the future. -
Lazzaro Spallanzani
He performed experiments in the 1760s, where he boiled different liquids, such as broth and water, in sealed flasks and showed that no microbial growth occurred unless the seal was broken. -
Louis Pasteur
This scientific, in order to demonstrate his theory against spontaneous generation, he does the next experiment: he exposed broths boiled in flasks provided with a filter that does impossible the entrance of dust and other flasks that had very large and curved necks that complicate the passage of dust particles, to the culture broth. After some time he observed that nothing grew, demonstrating that it wasn't spontaneous, if not it was generated probably from dust or in the form of spores. -
John Tyndall
This scientific directed a beam of light through a dark room filled with dust particles. By illuminating the room, he made the dust particles visible. Also, Tyndall collected air samples from various environments in order to cultivate the collected samples in nutrient media to identify and study the microorganisms present. With all of that, he proved the presence and the potential growth of microorganisms in the air.