John Needham put boiled nutrient broth into covered flasks
Lazzaro Spallanzani boiled nutrient solutions in flasks
Edward Jenner inoculated a person with cowpox virus.
Agostino Bassi showed that a silkworm disease was caused by a fungus
Ignaz Semmelwise advocated hand washing to prevent transmission of puerperal fever from one OB patient to another
Joseph Lister used a chemical disinfected to prevent surgical wound infections after looking at Pasteur's work showing microbes are in the air, can spoil food, and cause animal diseases
Louis Pasteur demonstrated that microorganisms are presented through the air
Pasteur believed that another silkworm disease was caused by a protozoan
Robert Koch proved that a bacterium causes anthrax and provided the experimental steps, Koch's postulates, to prove that specific microbe causes a specific disease
