Pregermination of cucumber seedlings: surface sterilization of seeds; incubate seeds on water agar in the dark
Grow biocontrol bacteria in liquid culture
Set up experiment: add pathogen to the soil, plant 3 pregerminated cucumber seedlings per pot and water pots with bacteria or control as well as with water
add pathogen to the soil, plant 3 pregerminated cucumber seedlings per pot and water pots with bacteria or control as well as with water
Period: to
Running of experiment
water plants
water plants
water plants
water plants
Harvest of experiment
assessing seedlings for survival/ scoring/ fresh weight