Biocontrol experiment

Grow P.ultimum on Malt Agar

Jun 20, 2012

Preparations

Period: Jun 20, 2012 to Jul 3, 2012

Grow P. ultimum on Sorghum

Jun 27, 2012

Grow biocontrol bacteria on agar plates

Jul 1, 2012

Pregermination of cucumber seedlings: surface sterilization of seeds; incubate seeds on water agar in the dark

Jul 2, 2012

Grow biocontrol bacteria in liquid culture

Jul 3, 2012

Set up experiment: add pathogen to the soil, plant 3 pregerminated cucumber seedlings per pot and water pots with bacteria or control as well as with water

Jul 4, 2012

add pathogen to the soil, plant 3 pregerminated cucumber seedlings per pot and water pots with bacteria or control as well as with water

Running of experiment

Period: Jul 4, 2012 to Jul 16, 2012

water plants

Jul 6, 2012

water plants

Jul 9, 2012

water plants

Jul 11, 2012

water plants

Jul 13, 2012

Harvest of experiment

Jul 16, 2012

assessing seedlings for survival/ scoring/ fresh weight