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Though United as one nation, the North and South were extremely different in cultur and Economy. The North was a very industrial area with no slavery, while the south was an agricultural economy, based of slavery and farming,
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The Missouri Comprimise doesn't seem as though it would be in the top three, but this is one of the most important, because it was the major problem that would soon rearise because it was not handled correctly
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This Rebelion was not very large because of the failure of slaves, though a few hundred people were killed, this was put down quickly and hushed up in a way
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This war created more states that would soon reignite the Missouri Comprimises failures, adding states like California that crossed the border
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This act was one of the last breaking points for the Northerners, for now even abolishionists were being forced to help slavers find there escaped.
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The border war between Kansas and Nebraska was an intense battle for choosing between a slave and free state. Though chosen as a slave state, it was untrue, because many wanted it to be a free.
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Evaluating that slave were not to be treated as people, but property, this case angered all abolishionists.
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John Brown's raid was trying to acquire weapons to help start a Civil War to help free the slaves, which infurriated the Southern slave holders
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This would be the first, though without the differences, this wouldn't be possible. the south Seceding basically was the cause of the war because of all the bottled up tension.
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The Firing on fort Sumter is not a major starting of the Civil War, it is just the tension finally releasing and unleashing the full fury of both nations hate.