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It is the first important work devoted to microscopical observation, and showed what the microscope could do for naturalists
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He did an aphorism called "omnis cellula e cellula" (every cell prom a pre-existing cell) and it became the basis of the theory of tissue formation.
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They allowed a more precise histological observations.
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Robert Brown, a Scottish botanist, introduced he term nucleus, and became the first to recognize the nucleus. He saw it as an essential constituent of living cells.
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He also was the indefatigable champion of the neuron theory.
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The new development of the microtome technique and the use of new fixing methods and dyes greatly helped improve microscopy.
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A German histologist, Joseph Gerlach, had expanded on Kolliker's view and proposed an idea. His idea was that in all of the central nervous systems, nerve cells have established anastomoses with one another through a network that was formed by the minute branching of their dendrites.
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The most important breakthrough in neurocytology and neuroanatomy history. A man named Camillo Golgi had developed the 'black reaction', which was introduced to a friend of his.
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The Swiss embryologist named Willhelm His had put his idea out that the nerve-cell body and its prolongations can form from an independent unit.
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Waldeyer introduced the term neurons to indicate independent nerve cells.