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The North had Industrialized, so there was less need for slave labor, while the South remained mostly agrarian. The economic future of the North did not require labor while the Southern economy did.
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Removed and Relocated of tens of thousands of Native Americans to new lands west of the Mississippi River to open up lands for White American settlers.
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After winning Texas from Mexico, it becomes the 28th US state, and a slave state. Its admission into the Union reheated the discussion of slavery.
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New lands beyond the Louisiana purchase bought, annexed, or otherwise brought into the U.S and later organized into states or Territories after winning an armed conflict against Mexico.
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The Supreme Court upheld slavery, denied black rights and citizenship and declared the Missouri Compromise unconstitutional.
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When conflict broke out in the Territories of Kansas and Missouri over the issue of whether or not the Territores of Kansas-Missouri should become Slave or Free states.
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Led by John Brown, in retaliation for the Sacking of Lawrence by Pro Slavery fighters in Bleeding Kansas, killed several Pro-Slavery settlers which only increased violence in these territories.
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Radical Abolitionist John Brown was fighting in Bleeding Kansas and led a failed slave revolt to seize weapons and ammunition from Harpers Ferry, VA.
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The Republicans were against slavery, everybody knew that. Having a Republican president in charge meant that the southern way of life may cease to exist. The South needed to protect their ways and rights, whatever the cost.
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The final major event before the Battle of Fort Sumter which initiated the Civil War. The southern states secede from the Union which prompted Lincoln to bring them back to the Union by any means.