• Francesco redi

    Francesco redi
    The procedure Francesco followed was to put raw meat in two jars, one open and the other closed. According to general belief, caterpillars would appear spontaneously in both jars. But in Francesco's experiment they only appeared in the open jar. And he showed that if that jar was closed at that moment, the caterpillars would turn into flies.
    Then those flies laid eggs and from those eggs the larvae emerged. These became flies, which in turn could lay eggs.
  • John Needham

    John Needham
    He destroyed any pre-existing organisms by boiling meat chunks and then put them in an improperly sealed container since, in his belief, air was necessary for this to happen. He eventually came to the conclusion that the colonies of microbes on the surface were the result of spontaneous generation from inanimate materials.
  • Lazzaro Spallanzani

    Lazzaro Spallanzani
    Lazzaro Spallanzani, was a biologist and at the same time a Catholic priest.
    Continuing Redi's study, Spallanzani showed that the spontaneous generation of life does not exist.
    In 1769, he designed experiments in order to refute the experiments carried out by John Tuberville Needham, who had heated and sealed meat broth in various containers.
    Spallanzani was able to demonstrate that those broths did not generate microorganisms as long as the containers were sealed.
  • Louis Pasteur

    Louis Pasteur
    He was a French microbiologist and chemist, who was known for his demonstration of the germ theory of disease and his development and inoculation techniques. Pasteur used containers with long and curved necks, in which he placed a broth that had boiled for a few minutes. When removing it from the fire, the air entered through the neck, but the microbes were trapped in it, which prevented them from contaminating the liquid and allowed it to be kept sterile indefinitely.