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Enzymes were discovered as early as the 18th or 19th century, but a man named Eduard Buchner developed more into the theory of enzymes and was given the Nobel Peace Prize in 1907. -https://en.wikipedia.org/wiki/History_of_biochemistry#Enzymes
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In the first decade of the 20th century, chromatography was invented. Chromatography is a laboratory technique for the separation of a mixture. -https://en.wikipedia.org/wiki/Chromatography
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X-ray crystallography is a technique used for determining the atomic and molecular structure of a crystal. -https://en.wikipedia.org/wiki/X-ray_crystallography
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An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. -https://en.wikipedia.org/wiki/Electron_microscope
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NMR Spectroscopy is a spectroscopic technique to observe local magnetic fields around atomic nuclei. https://en.wikipedia.org/wiki/Nuclear_magnetic_resonance_spectroscopy_of_proteins
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Radioisotopic labeling is a technique used to track the passage of an isotope (an atom with a detectable variation in neutron count) through a reaction, metabolic pathway, or cell. -https://en.wikipedia.org/wiki/Isotopic_labeling
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Before the 20th century, not a lot was known about Metabolism. But a biochemist named Hans Krebs made huge discoveries on metabolism and was awarded the Nobel Peace Prize in 1953. https://en.wikipedia.org/wiki/History_of_biochemistry#Metabolism:_20th_century_-_present
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Molecular dynamics (MD) is a computer simulation method for studying the physical movements of atoms and molecules. -https://en.wikipedia.org/wiki/Molecular_dynamics
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Polymerase chain reaction (PCR) is the primary gene amplification technique that has revolutionized modern biochemistry. https://en.wikipedia.org/wiki/History_of_biochemistry#Metabolism:_20th_century_-_present
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The Thermal Cycler is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). -https://en.wikipedia.org/wiki/Thermal_cycler
