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A Dutchman by the name of Antoni van Leeuwenhoek discovered the movement of microorganisms. He then assumed that movement equates to life, he verified this by writing a letter to the Royal Society on October 9 1676, that the organism he seen were indeed living.
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First official published work of microscopical observation of any kind was released by a man named Robert Hooke. He informed that at that level you can see the building blocks of the cell structure.
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Matthias Jakob Schleiden suggested that all structural elements of plants is composed of cells because of the research of cells including similarities.
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Cell Theory was formulated, when before cells were not considered or seen as a different structure. They have visualized the nucleus and other few cellular components in cells which gave them idea of that they are living matter, since they were seen in many different cells with other similarities.
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The Spanish neuroanatomist Santiago Ramon y Cajal became the main supporter of the neuron theory. His investigations contributed to the basic foundations of the neuron theory. Even though defiant proof of neuron theory obtained later.
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Microtome technique used oil and dyes for fixing and very much improved microscopy.
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More in-depth understanding that was proposed by Joseph Gerlach that nerve cells established a connection to the nervous system through a network formed by the minute branching of their dendrites They assumed this structure together was an essential for anatomical and functional communications.
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Wilhelm His was expressing the idea that the nerve cell body and its long lasting form an independent unit.