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He published the monograph “Ueber die Parasiten der Malaria” in Zentralblatte für Bakteriologie und Parasitenkunde (Grassi & Feletti, 1890, 1891a, b), in which he described the malarial cycle in different species of birds, such as owl, pigeon, and sparrow.
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In addition, it was stated that the findings corresponded to what Ross had reported for Proteosoma in Culex pipiens in the malarial cycle of birds.
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The article consisted of 200 large-format pages in which Grassi summarized the four years of research, from 1896 to 1899, and underlined the originality of his contribution by defining himself as a zoologist.
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