-
1.搜集整理官网上已有的降解肽（如LVA等）及其相关信息，包括biobrik编号，是否可用，竞赛委员会是否会寄给我们，experience数据等等（2人）
2.搜集整理官网上尚未注册，但是各种文献中出现过的降解肽类物质信息，包括作用、机制、如何获取等等（2人）
3.mRNA半衰期问题，如何提高或降低其半衰期，机制，提出可行方案（2人）
4.整理大家的资料，presentation展示（1人）
注：请一定注明参考文献和网址相关链接~ -
Because of the short of experience,the useful information is not enough in quality and quatity.
-
Discuss with orthers in our team about the conclusion and solve problems.
Prepare for the presentation in 7:00 p.m. -
All team members participate in the meeting.
1. BGILL & Krystal_CN : find out useful degration tags in iGEM distribution kit 2013;
2. kolar_mr & zyllei & Rosa_rubu :make a
series of N-end and C-end degration tags,
design oligos for synthesizing these
BioBricks, or PCR primers to get them from
yeast or E. coli. Discuss the design of ubiquitin related BioBricks;
3.iaofu&fishWR： discuss engineer mRNA half life related elements.Sarch for miRNAs and reflect half life of specific
transcripts. -
all of us do some survey and design
-
-
find the half-life of cell cycle protein in
Yeast and some protein in E. coli to confirm
the theory of N rule -
The degradation peptides of Saccharomyces
cerevisiae cyclins Contact WHU igemers and
find out the cyclins in Saccharomyces
cerevisiae.Find out degradation peptides which
are realted with cyclins and identify
mechanisms of protein degradation -
To find out the molecular principles of N-
end rule pathway , and confirm the rule can be
used in our projuct . -
Measure fluorescent protein's half-life
(considering feasibility issues)and contact
SCU to get the fluorescent protein. -
the transformation of plasmid into yeast
about theory and method of the experiment
,selection of plasmid skeleton and the
position of plasmid in yeast, cytoplasm or
nucleus -
Search for standardized promotors kits on
iGEM official website to determine which one
to be used in our project. Information about
RBS should be taken in consideration. -
design the experiment of synthetic 3 kits we need in detail and the of the frame of whole project of our part.
-
Search for destruction box fit for our project on iGEM official website and design primers for synthetic
-
continue to find more half life of the cell cycle protein, to identify the N-end rule.and find some paper about the synchronization by using microfluidic platforms
-
1.to confirm the molecular mechanism of ubiquitin degradation and N-end pathway
2. to check whether the design of prime is resonable and whether the N-end primer can be synthetized -
1、 Find out proteins with PEST sequences which are rich in Pro (P), Glu (E), Ser (S) and Thr (T) and more rapidly degraded than other proteins from the Official website of IGEM
2、 Identify the most appropriate PEST sequence according to half-life of proteins that we have found
3、 Design primers of PEST sequence when we confirm what we have done is reliable -
Measure protein half-life by Microfluidic method and find other feasible half-life measurement method.
-
1、Search for theories and experiment methods for YEp352,YEplac195 and YEplac181.2、design transformation experiment of S.cerevisiae by electroporation
-
read the paper and identify the methods of microfluidic chip in cell cycle synchromization exactly.
-
To comfirm the molecular mechanism of n-end pathway rule again and check the accuracy of primers carefully.
-
search for cyclins to validate N-rule
-
determine the method to measure the protein half-life